Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Research Products International Corp TES, Sodium Salt, 1 KG
TES (N-[Tris(hydroxymethyl)methyl]-2-aminoethanesulfonic acid) Sodium Salt is a commonly used buffering agent in life science research. It helps to maintain a stable pH environment in biological experiments, such as cell culture, protein purification, and enzyme assays.
TES Sodium Salt is advantageous due to its ability to resist changes in pH over a wide range of temperatures, making it suitable for various experimental conditions.
Additionally, its low UV absorbance and minimal interference with biological molecules make it a preferred choice in many biochemical applications
Revvity Health Sciences Inc HTRF PPI Terbium detection buffer, 220 ml
HTRF PPI Terbium detection buffer, 220 ml
Research Products International Corp Manganese Chloride 1M Solution, 100 ML
Manganese Chloride is used for dC and dG tailing of DNA.
Seracare Life Sciences Inc 10X TRIS BUFFERED SALINE (TBS)
10X Tris-buffered Saline (TBS) is used as a general wash buffer in immunoassay applications.
Gold Biotechnology Inc HEPES, Free Acid 250 g
HEPES is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, piperazinic buffer that is useful for a pH range of 6.8 – 8.2. HEPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. It is commonly used in cell culture media and as an ampholytic separator to create a pH gradient in isoelectric focusing. HEPES interferes with reactions between DNA and restriction enzymes to a lesser extent than similar buffers with less substituted amine groups, such as Tris. This lack of interference occurs because steric hindrance of the protonated tertiary amine in HEPES shields the negative charge of DNA to a lesser extent than less substituted buffers. This buffer is capable of forming radicals and is therefore not suitable for redox reactions. It is suitable for use in the bicinchoninic acid (BCA) assay.
Sigma Aldrich Fine Chemicals Biosciences MOBS >=99% (titration) | 115724-21-5 | MFCD00674916 | 100G
MOBS >=99% (titration) | Purity: >=99% (titration) | Mol Wt: 223.29 | 115724-21-5 | MFCD00674916 | 100G
Research Products International Corp HEPES, Sodium Salt, 5 KG
pKa (20°C): 7.45-7.65
pH Range: 6.8-8.2
![Research Products International Corp RPI MOPS [3-(N-morpholino)-propanesulfonic acid], 10 Kilograms](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M92020-10000.0.JPG-250.jpg)
Research Products International Corp RPI MOPS [3-(N-morpholino)-propanesulfonic acid], 10 Kilograms
MOPS, or 3-(N-Morpholino) propanesulfonic acid, is a zwitterionic buffer. MOPS is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 6.5 to 7.9, making it suitable for applications requiring slightly acidic to neutral pH conditions.
MOPS is commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of MOPS is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes, ensuring reliable and accurate results.
pH Range: 6.5-7.9
Seracare Life Sciences Inc 10X DULBECCO S PBS (D-PBS)
10X Phosphate-buffered Saline (PBS) is ideal for use in sample preparation and as a wash buffer in general immunoassay applications as well as tissue and cell culture procedures. This modified Dulbecco's buffer is not formulated with magnesium or calcium salts. pH=7.4. Conductivity controlled.
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 7.8) - 1L
Tris-HCl Buffer (1 M pH 7 8) - 1L (For Research Use and Further Manufacturing Only)
LAB BIOREAGENTS NGB II Buffer 100ml
Lab Bioreagents NGB (I, II, III) are newly developed next generation assay buffers that provides exceptional performance and broad compatibility for Western blot, ELISA, IHC and nucleic acid detection assays. The NGBs are basic buffers that hat requires neutralization by acid in the reaction. Among the tests, NGBs have been demonstrated to maintain the full bioactivity of proteins and antibodies.
S2 Media 100mL 1x Phosphate Buffered Saline (PBS), pH 7.4, 125mL Media Bottle, 1ea
1x Phosphate Buffered Saline (PBS), pH 7.4 has many applications due to its isotonic nature, including uses as a rinse fluid, diluent, and buffer. Phosphate salts are nontoxic to living cells and have a high buffering capacity. pH maintenance is important in retaining cell viability and in the recovery and revitalization of injured or damaged cells during bacterial culture and analysis. PBS is generally used as a 10mM working solution containing a mixture of sodium chloride and inorganic phosphate salts.• Product meets AOAC, APHA, BAM, CLSI, SM-Dairy, SMWW, and USP-NF reference methods and performance criteria.• For in vitro diagnostic (IVD) use.• PBS should be used by trained professionals within a laboratory setting. • Product is not intended for use in the diagnosis or treatment of disease or other human conditions.
Biotang Inc Sodium Phosphate Buffer 0.5M, pH7.6, 250 ml
Sodium Phosphate Buffer 0.5M, pH7.6, 250 ml.
Boston Bioproducts Inc Tris-EDTA Buffer, TE (20X, pH 8.0) - 250ml
The Tris-EDTA (TE) Buffer is a commonly used buffer solution in molecular biology, especially in processes involving nucleic acids. This buffer is composed of a 20X concentration of Tris, a buffering agent, and EDTA, which has the ability to prevent degradation of DNA and RNA by chelating magnesium or other divalent metal ions, and allows pH regulation within the range of 7.5-8.5. It is widely used often for DNA and RNA gel electrophoresis, and also for DNA automated sequencing.