Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Bioworld TBE Buffer, Quick-Mix Pack (25X), 2 PK(s)
Product DescriptionTBE (Tris-Borate-EDTA) quick-mix pack prepares 1L of 25X concentrate. Each pack contains 0.089M Tris, 0.089M Borate, and 0.002M EDTA. Tris solutions are effective buffers for slightly basic conditions. Borate, a derivative of boric acid, is a weak proton donor but a Lewis acid that can accept an electron pair. EDTA is a chelator of divalent cations, mainly Mg2+. TBE is most commonly used for nucleic acid electrophoresis.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.
![Alkali Scientific MOPS, Sodium Salt [3 (N Morpholino) propanesulfonic acid sodium salt], 500 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660144.jpg-250.jpg)
Alkali Scientific MOPS, Sodium Salt [3 (N Morpholino) propanesulfonic acid sodium salt], 500 G
MOPS (3-(N-Morpholino)propanesulfonic acid sodium salt) is a highly effective pH buffer, widely used in biochemical and biological research. This 500g volume provides a sufficient quantity for larger scale experiments or laboratories that regularly require buffer solutions. MOPS is most commonly utilized in protein and nucleic acid studies, particularly where consistent pH control is necessary over extended periods. It ensures that the pH remains stable within the ideal range of 7.0–7.5, which is critical for maintaining optimal conditions in enzyme assays, electrophoresis, and cell culture experiments. It is also frequently used in molecular biology, biotechnology, and clinical research protocols.
Bioworld TBE Sample Buffer (4X), 25 mL
Tris-Borate Buffer, EDTA (TBE) Sample buffer for nucleic acid analysis. Technical Information: 4X concentration Tris base (270mM) Boric Acid (270mM) Glycerol (36%) Bromophenol blue G (0.025%) Xylene Cyanol (0.025%) EDTA (6mM)
ILEX LIFE SCIENCES LLC TRYPSINEDTA P10-024100 6X100ML
PAN-Biotech (10x) Trypsin 0.5% / EDTA 0.2% in DPBS, w/o: Ca and Mg (6 x 100 ml). DESCRIPTION: PAN-Biotech (10x) Trypsin 0.5% / EDTA 0.2% in PBS, w/o: Ca and Mg is a porcine pancreas-derived enzyme plus EDTA in a 10x concentrated PBS solution. Trypsin-EDTA solution is commonly used for the dissociation of anchorage-dependent (adherent) mammalian cells and tissues from culture surfaces. The concentration of trypsin necessary to dislodge cells from their substrate is dependent on the cell type and the age of the culture. Ethylenediaminetetraacetic acid (EDTA), a chelating agent, is often added to trypsin solutions to enhance enzymatic activity by neutralizing calcium and magnesium ions that enhance cell-to-cell adhesion and obscure the peptide bonds on which trypsin acts. LIQUID/POWDER: Liquid. PHENOL RED: Without Phenol Red. Ca/Mg CONTENT: Without Ca and Mg. TRYPSIN: 0.50% Trypsin. EDTA: 0.20% (5.3 mM) EDTA. STERILE: Yes. STORAGE TEMP.: -20°C. MANUFACTURER: PAN-Biotech GmbH (Germany).
Bioworld BES Buffer, 0.5M, pH 7.5, 100 mL
Product DescriptionBES (2-(Bis(2-hydroxyethyl) amino) ethanesulfonic acid) buffer has a concentration of 0.5M and a pH value of 7.5. BES is a zwitterionic buffer used in biochemistry and molecular biology, and has been used to study versatile catalyst precursors for mild hydrocarboxylation of alkanes to carboxylic acids. BES was used to determine that saline buffers minimize the action of interfering factors in the bacterial endotoxins test.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.
Alkali Scientific Murashige & Skoog Medium with MES Buffer and Vitamins, 24.5 Grams of Powder, Makes 5 Liters of Solution
Murashige & Skoog Medium with MES Buffer and Vitamins (5 liters, 24.5 grams of powder) is used for plant tissue culture applications that require precise control over pH levels and nutrient availability. The MES buffer stabilizes the pH environment, ensuring that plant cells receive consistent growth conditions. This medium is ideal for a variety of tissue culture applications, including shoot multiplication, regeneration, and plant propagation. The formulation includes essential vitamins and nutrients that support plant growth, making it an essential tool for researchers, plant breeders, and commercial growers. It is commonly used in agricultural laboratories and greenhouses to produce high-quality, healthy plant cultures.
Sigma Aldrich Fine Chemicals Biosciences MOPS hemisodium salt >=99% (titration) | 117961-20-3 | 100G
MOPS hemisodium salt >=99% (titration) | Purity: >=99% (titration) | Mol Wt: 220.25 | 117961-20-3 | 100G
ILEX LIFE SCIENCES LLC GELATIN SOLUTION 2.0 6 X 100ML
PAN-Biotech Gelatin Solution 2% in DPBS, w/o: Ca, Mg (6 x 100 ml). DESCRIPTION: PAN-Biotech Gelatin Solution 2% in DPBS, w/o: Ca, Mg for laboratory research applications. Gelatin is a heterogenous mixture of water soluble proteins of high average molecular weight present in collagen. The proteins are extracted by boiling skin, tendons, ligaments, or bones in water. APPLICATION: Applications using gelatin include coating cell culture plates to improve cell attachment for a variety of cell types, addition to PCR to stabilize Taq DNA Polymerase, and use as a blocking agent in Western Blotting, ELISA, and immunohistochemistry. In bacteriology, gelatin can be used as a component of culture media. STORAGE: +2-8°C. MANUFACTURER: PAN-Biotech GmbH. COUNTRY OF ORIGIN: Germany.
ILEX LIFE SCIENCES LLC GELATIN SOLUTION 0.1 6X500ML
PAN-Biotech Gelatin Solution 0.1% in DPBS, w/o: Ca, Mg (6 x 500 ml). DESCRIPTION: PAN-Biotech Gelatin Solution 0.1% in DPBS, w/o: Ca, Mg for laboratory research applications. Gelatin is a heterogenous mixture of water soluble proteins of high average molecular weight present in collagen. The proteins are extracted by boiling skin, tendons, ligaments, or bones in water. APPLICATION: Applications using gelatin include coating cell culture plates to improve cell attachment for a variety of cell types, addition to PCR to stabilize Taq DNA Polymerase, and use as a blocking agent in Western Blotting, ELISA, and immunohistochemistry. In bacteriology, gelatin can be used as a component of culture media. STORAGE: +2-8°C. MANUFACTURER: PAN-Biotech GmbH. COUNTRY OF ORIGIN: Germany.
![Alkali Scientific HEPES, Sodium Salt [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 500 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659775.jpg-250.jpg)
Alkali Scientific HEPES, Sodium Salt [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 500 G
HEPES, Sodium Salt is a biochemical reagent available in a 500g size, ideal for large-scale applications in biological and chemical research. It is widely used to maintain pH stability in biological systems, particularly in cell culture, protein assays, and enzyme reactions. HEPES sodium salt provides reliable buffering capacity in the pH range of 6.8–8.2, ensuring that experimental conditions remain consistent. It is non-toxic to cells, supporting reproducible results in long-term experiments. This bulk package is perfect for high-throughput laboratories, where large quantities of buffer are required for routine cell culture and biochemical analysis procedures.
Apexbio Technology LLC Annexin V-FITC/7-AAD Apoptosis Kit 20assays
Annexin V is a calcium-dependent phospholipid-binding protein that specifically binds phosphatidylserine (PS) Normally PS is localized to the inner leaflet of the cell membrane lipid bilayer but during early stages of apoptosis PS flips from the inner leaflet to the outer surface of the plasma membrane where it is exposed to the extracellular environment Annexin V displays a high affinity for PS Fluorescein (FITC)-conjugated Annexin V can be used as a fluorescent probe in flow cytometry or fluorescent microscopy to detect apoptosis within 10-20 minutes The nuclear dye 7-Aminoactinomycin D (7-AAD) specifically binds to double-stranded DNA and fluoresces upon binding Under normal circumstances 7-AAD is cell-impermeable however in late apoptotic and dead cells 7-AAD can pass through cell membranes staining DNA Combining Annexin V-FITC with 7-AAD staining cells at different stages of apoptosis and dead cells can be distinguished effectively
Medchemexpress LLC P62-mediated mitopha 10mM 1mL | 1809031-84-2 | 392.15 g·mol⁻1 | C14H9IN4O2 | 1 ML
P62-mediated mitophagy inducer is a small-molecule research reagent that activates p62-mediated mitophagy without recruiting Parkin or collapsing mitochondrial membrane potential. It is supplied as a 10 mM stock solution (1 mL) for use in cellular and biochemical studies of selective mitochondrial clearance and autophagy pathways.
- Activates p62-mediated mitophagy without Parkin recruitment.
- Provided as a ready-to-use 10 mM stock solution in 1 mL.
- Suitable for cellular and biochemical assays investigating mitophagy and autophagy.
- Stable storage recommendations: powder at -20°C for up to 3 years; in solvent at -80°C or -20°C as indicated.
- Comes with chemical identification and characterization data (CAS, formula, molecular weight) in the supplier datasheet.
![Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 50 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659758.jpg-250.jpg)
Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 50 G
HEPES, Free Acid is a critical biochemical reagent for maintaining pH stability in cell cultures and biochemical experiments. This 50g package is ideal for smaller scale applications, where precise pH control is essential for the success of experiments. HEPES is effective in a pH range of 6.8 to 8.2, which makes it a preferred choice for a variety of applications including enzymatic assays, electrophoresis, and cell-based assays. Due to its excellent buffering capacity and low toxicity to cells, HEPES ensures that pH variations are minimized during extended incubation periods, supporting reliable and reproducible experimental outcomes.
![Alkali Scientific MOPS, Sodium Salt [3 (N Morpholino) propanesulfonic acid sodium salt], 1 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660034.jpg-250.jpg)
Alkali Scientific MOPS, Sodium Salt [3 (N Morpholino) propanesulfonic acid sodium salt], 1 Kg
MOPS (3-(N-Morpholino)propanesulfonic acid sodium salt) is a pH buffering reagent commonly used in biological and chemical research. This 1 kilogram bottle provides a larger quantity of MOPS sodium salt for high-volume applications requiring consistent pH stability. It is ideal for maintaining physiological pH in biochemical and biological experiments, such as enzyme assays, protein analyses, and cell culture maintenance. The reagent is particularly useful in experiments that involve prolonged reaction times, as it ensures that pH remains within a narrow, optimal range for sensitive biological systems. MOPS is also used in molecular biology for electrophoresis and other lab protocols that require precise pH control.